Please note: The Research + Innovation office will be closed for the holidays from noon on December 24 through January 2nd, inclusive. 鉂勶笍 Veuillez noter que le bureau de la Recherche et de l'Innovation sera ferm茅 pour les vacances du 24听d茅cembre 脿 midi jusqu鈥檃u 2听janvier inclus.
麻豆区
On this page:听Adding a new individual to a protocol听| Animal methodology workshop Information |听Training Opportunities听
To ensure ethical and compliant practices, we kindly ask all 麻豆区 staff and students working with animals to register for the Animal Compliance Office session. During this session, we will guide you through the necessary processes and steps involved in working with animals at 麻豆区. Please note that this session is exclusively for 麻豆区 staff and students.
The 麻豆区 mandatory training involves:
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Theory course: Everyone working with animals must successfully pass听麻豆区's听online course.
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Practical training: All personnel who will be handling live rodents and rabbits need to take hands-on workshops given at one of our several training sites (more information below).
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For practical training with avian species, farm animals, reptiles, amphibians and fish, please contact animalcare [at] mcgill.ca for more information.
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Farm Course: Livestock Handling Theory portion
Adding a new individual to a protocol / What steps should I follow to work with animals?
- Get access to the Animal Management Software听
- Register to the appropriate species-specific modules and training sessions covering the procedures you will perform. Register as soon as possible since classes fill up quickly.
- For the theory portion, click here to read the material online.
- Ask your PI to add you to their Animal User Protocol.
- Request a Facility Orientation.
After the orientation is complete, you may now be granted access to the facility.
For species other than laboratory rodents and rabbits, the person will need to have received training from an experienced person (who must meet approval by the Animal Care Committee). A form called 'Certification for Non-rodent Non-rabbit Training' must be completed by the trainer and submitted to the animalcare [at] mcgill.ca email. A student evaluation form is also required where the student initials after they have read the species-appropriate SOPs and their Animal Use Protocol. The forms to complete are available by emailing the animalcare [at] mcgill.ca (subject: Form%20Request%3A%20Certification%20for%20non-rodent%2C%20non-rabbit%20training%20) (Training Advisor).
More information on the CCAC training requirements can be obtained at: .
To inquire if specific specialized training is available, please contact the animalcare [at] mcgill.ca (subject: Specialized%20Training%20Availability) (Training Advisors).
Animal methodology workshop Information - What training do you need?
Technique | Description | Training Information |
---|---|---|
Blood Collections 听 |
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Cardiac Puncture |
Terminal procedure. See Isoflurane followed by physical method. |
See Isoflurane followed by physical method. |
Jugular Vein |
Drawing blood from the jugular vein in the neck |
Mice: Taught in specialized training, modules 1 and 3 are prerequisites. 听 Module 2 is required if using an injectable. 听 Rats: Taught in specialized training, module 1 is a prerequisite. |
Retro-orbital (justify) |
Drawing blood from the orbital sinus located behind the eye |
Taught in specialized training, module 1 is a prerequisite. |
Saphenous Vein |
Drawing blood from the lateral saphenous vein in the hind leg |
Taught in module 7, module 1 is a prerequisite. |
Submandibular |
Drawing blood from the submandibular vein, located beneath the jaw |
Taught in module 7, module 1 is a prerequisite. |
Tail Puncture |
Collecting blood using the point of a blade/needle to puncture the vessel |
Taught in specialized training, module 1 is a prerequisite. |
Tail Snip |
Collecting blood by transecting (cutting off) portion of mouse tail under anesthetic and analgesia. Repeat samples can be taken by removal of the scab. |
Taught in specialized training, modules 1 and 3 are prerequisites. |
Tail Vein |
Drawing blood from the coccygeal venipuncture in the tail |
Taught in module 7, module 1 is a prerequisite. |
Injections | ||
Intradermal (Intraplantar, Footpad...) |
Administered into the dermal layer of the skin |
Taught in specialized training, module 1 is a prerequisite. |
Intrafemoral |
Administered into the femoral bone |
Taught in specialized training, modules 1,2 and 3 are prerequisites. |
Intramuscular |
Administered into the muscular tissue |
Mice: Taught in specialized training, module 1 is a prerequisite. Rats: Taught in module 2, module 1 is a prerequisite. |
Intranasal (Instillation) |
Administered through the nasal passages |
Requires passing module 1. Unless performed under anesthesia, which requires module 3 |
Intraperitoneal |
Administered into the peritoneal cavity |
Taught in module 2, module 1 is a prerequisite. |
Intratracheal (Instillation) |
Administered directly into the trachea |
Taught in specialized training, modules 1 and 3 are prerequisites. |
Intratibial |
Administered into the tibia (shinbone) |
Taught in specialized training, module 1 is a prerequisite |
Intracranial (Neonates only) |
Administered into the brain |
Requires modules 1, 2 and 3. |
Intravenous/ Tail vein |
Administered into the vein via the tail |
Taught in module 6, module 1 is a prerequisite |
Subcutaneous |
Administered beneath the skin |
Taught in module 2, module 1 is a prerequisite. |
Retro-Orbital (justify) |
Administered into orbital sinus behind the eye |
Taught in specialized training, module 1 is a prerequisite |
Via Cannulation |
Administered into a surgically implanted tube |
Requires module 1. |
Via Tumour or Abscess |
Administered either into tissue surrounding the tumour or abscess or directly into it. |
Surrounding tissue: Requires modules 1 and 2 (for subcutaneous). 听 Direct: Requires module 1 |
Oral Gavage |
Administered into the esophagus via mouth using a feeding tube or needle |
Taught in specialized training, module 1 is a prerequisite |
Other Procedures | ||
Handling and Restraint |
Holding and immobilizing the animal |
Requires module 1 |
Behavioural Testing |
Testing through using behavioural apparatus |
Requires module 1 |
Breeding Colony Management |
Mating and weaning animals |
Taught in module 5 (optional), module 1 is a prerequisite |
Intubation |
Survival procedure: Insertion of a tube into the trachea |
Taught in specialized training, modules 1 and 3 are prerequisites |
Implantation |
Placement of a device or substance into the body |
Requires modules 1,2 and 3 |
Rectal Administration |
Introduction of substances through the rectum |
Requires module 1 |
Genotyping - Ear Punch |
Punching a small hole in the ear of an animal |
Demonstration in module 1 |
Genotyping 鈥 Fecal Pellet |
Collection of fecal pellets |
Requires module 1. |
Genotyping 鈥 Tail Tip |
Transecting (cutting off) a small portion of a rodent's tail |
Under 21 days of age: Requires module 1. Over 21 days of age: Requires modules 1,2 and 3. |
Genotyping 鈥 Toe Tip |
Transecting a small portion of a young rodent鈥檚 toe (under 7 days of age) |
Taught in specialized training, module 1 is a prerequisite. |
Identification 鈥 Ear Punch/Ear Notch |
Punching a small hole in the ear of an animal for identification purposes |
Demonstration in module 1. |
Identification 鈥 Micro-tattoo |
Identification of animals through tattoo with a small gauge needle or micro-tattoo |
Requires passing module 1, specialized training by request. |
Identification 鈥 Tattoo |
Identification of animals through tattoo |
Requires passing modules 1 and 3, specialized training by request. |
Identification 鈥 Microchip |
Identification of animals through subcutaneous implant of microchip |
Requires passing modules 1,2 and 3, Optional specialized training by request. |
Isoflurane and CO2 followed by Secondary Physical Confirmation (Decapitation, Pneumothorax, Cervical Dislocation, Cardiac Puncture) |
Isoflurane inhalant anesthetic followed by CO2 euthanasia, confirmed by a secondary physical method |
Taught in module 1 |
Isoflurane followed by exsanguination (Justify) |
Isoflurane inhalant anesthetic followed by severing major vein and/or artery resulting in euthanasia by blood loss |
Taught in specialized training, module 1 is a prerequisite. |
Isoflurane followed by physical method (Decapitation, Cervical Dislocation, Cardiac Puncture) |
Isoflurane inhalant anesthetic followed by physical method of euthanasia |
Requires passing Module 1 and Module 3 |
Anesthetic overdose followed by a secondary physical method (Decapitation, Cervical Dislocation, Cardiac Puncture) Isofluorane overdose followed by cervical dislocation. |
Euthanasia by anesthetic or barbiturate cocktail overdose, confirmed by a secondary physical method |
Requires passing modules 1 and 2 to perform. Specialized training may be required if the drug is not administered Intraperitoneally. Inhalant anesthetic overdose requires module 3. |
Bell Jar Method of euthanasia using Isoflurane followed by cervical dislocation (Justify) |
A closed receptacle containing cotton or gauze soaked with Anesthetic, followed by a physical method of euthanasia |
Requires passing module 1. |
CO2 chamber by a secondary physical method (Decapitation, Pneumothorax or Cervical Dislocation) (justify) |
CO2 euthanasia confirmed by a secondary physical method |
Requires passing module 1. |
Cervical Dislocation (without Anesthesia) 听 |
Euthanasia by cervical dislocation |
Taught in specialized training, module 1 is a prerequisite. |
Decapitation (without Anesthesia) |
Euthanasia by decapitation |
Taught in specialized training, module 1 is a prerequisite. |
Perfusion, Terminal (with Anesthesia) |
Isoflurane inhalant or injectable anesthetic followed by severing the right atrium and passing fluid through the lymphatic system or blood vessels to an organ or a tissue. |
Requires passing Module 1 and 3. 听 Module 2 is required if using injectable anesthetic |
Decapitation (with Anesthesia) for neonates |
Isoflurane anesthetic inhalant followed by decapitation with scissors for pups under 14 days |
Module 1 is required |
Decapitation (without Anesthesia) for neonates |
Decapitation with scissors for pups under 14 days |
Demonstration in Module 1 |
Decapitation (with Hypothermia Anesthesia) for neonates |
Using ice to induce hypothermic anesthesia followed by decapitation with scissors for pups under 7 days |
Requires passing Module 1 |
Anesthesia 鈥 Inhalant |
Induction and maintenance of anesthesia using an inhalant machine. |
Taught in module 3, module 1 is a prerequisite and in most cases module 2. |
Anesthesia 鈥 Injection |
Induction and maintenance of anesthesia using an injectable cocktail. |
Demonstrated in module 3, modules 1 and 2 are prerequisites. |
Anesthesia 鈥 Rapid Cooling (Neonates only) |
Induction and maintenance of anesthesia through hypothermia of neonatal rodents. |
Requires passing module 1 to perform. |
Any Surgical Procedure |
Any technique listed under the Study Segments section of the AUP. |
Taught in module 4 (optional). Requires modules 1,2 and 3 |
听
Training Opportunities/Continuing Education
- CCAC guidelines - Animal Welfare Assessment听 (Version听fran莽aise:听
- FACC Roles and Responsibilities - June 27 2023听
- CCAC - Reportable Animal Welfare Incidents听()
- CCAC guidelines - FACC site visits
- CCAC Guidelines - Wildlife
- CCAC Guidelines - Identification of Scientific Endpoints - Humane Intervention Points and Cumulative Endpoints
- :听November 17-20, 2024听Seattle, WA
- :听CALAS's annual symposium is a networking and education event for the Canadian Laboratory Animal Science community.
- Submission deadline: January 15th (oral presentations); January 22nd (poster presentations),听
Location: Saskatoon, Saskatchewan from June 22-25, 2024
- Submission deadline: January 15th (oral presentations); January 22nd (poster presentations),听
- Refined:听Learn why & how to use refined methods to pick up mice.
- ICCVAM Public Forum: May 2023:听
- Chinchilla Consortium:听Evidence-based refinements for
- Animal Research Communications Seminar and Workshops:听
- Care-full Stories: Interactive Training Resources for Developing Your Institution's :听Care-full stories is a set of training materials designed to encourage people working in animal research to reflect on their institution's culture of care.
- Zebrafish husbandry education courses sponsored by the Zebrafish Husbandry Association (ZHA) are now available at the University of (UAB).
- Focus on Fish:听The RSPCA has announced their free Focus on 听(available to to view anytime!)
- Tech :听NC3Rs has published a new issue of their Tech 3Rs听newsletter. They highlight resources to improve mouse welfare in high-yield behavioral experiments, showcase rat refinement, and discuss career paths.
- Focus on :听The RSPCA held their听4th international 鈥楩ocus on Severe Suffering鈥 meeting on August 2022. The meeting summary report is now available.
- , what is it and how to get help?听
- , a recorded webinar.
- , an interesting webpage.
- Canadian Aquatic Research Facilities SharePoint Site听
- The goal of this group is to provide a mechanism for Aquatic Facility Managers, Researchers, and Technicians to communicate with colleagues across Canada.
- To request membership, please submit your request to听alicia.oneill [at] ec.gc.ca.